Research assay kit LS-K302

for drug discoveryglyceroltriglycerides

Triglyceride , also known as Triacyltriglyceride or Triacyl-glyceride, is the main constituent in vegetable oil and animal fats. Triglycerides play an important role as energy sources and transporters of dietary fat. In the human body, high levels of triglycerides in the bloodstream have been linked to atherosclerosis, heart disease and pancreatitis. Simple, direct and automation-ready procedures for measuring triglyceride concentrations find wide applications in research and drug discovery. This triglyceride assay uses a single Working Reagent that combines triglyceride hydrolysis and glycerol determination in one step, in which a dye reagent is oxidized to form a colored product. The color intensity at 570nm is directly proportional to triglyceride concentration in the sample. Specifications Type Detection/Quantition Usage For quantitative determination of triglyceride and evaluation of drug effects on triglyceride metabolism. Target Triglyceride SampleType Plasma, Serum Detection Colorimetric (570 nm Absorbance) Supplied Components The following components are supplied with this product. Assay Buffer ATP Dye Reagent Enzyme Mix Lipase Standard (equivalent to 100 mmol /L Triglyceride) (See Datasheet for specific volumes supplied) Applications Spectrophotometry (visible) Equipment Microplate spectrophotometer (visible) Conditions Shipped -20°C Dry Ice, Store at -20°C, 3 months shelf life.