LDH assay kit LS-K306

for researchfor drug discoveryNAD

ACTATE DEHYDROGENASE (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. When disease or injury affects tissues containing LDH, the cells release LDH into the bloodstream, where it is identified in higher than normal levels. Therefore, LDH is most often measured to evaluate the presence of tissue or cell damage. The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the purple color formed is directly proportional to the enzyme activity. Specifications Type Detection/Quantition Usage For quantitative determination of lactate dehydrogenase LDH activity and screen/evaluation of LDH modulators. Target Lactate Dehydrogenase Detection Colorimetric (565 nm Absorbance) Supplied Components The following components are supplied with this product. Substrate Buffer Diaphorase NAD Solution Calibrator MTT Solution (See Datasheet for specific volumes supplied) Applications Spectrophotometry (visible) Equipment Microplate spectrophotometer (visible), Spectrophotometer (visible)