Alkaline phosphatase assay kit LS-K301

for researchserumplasma

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis. Simple, direct and automation-ready procedures for measuring ALP activity in serum are becoming popular in Research and Drug Discovery. This improved Method utilizes 4-methylumbelliferyl phosphate that is hydrolyzed by ALP into a highly fluorescent product 4-methylumbelliferone. The rate of the fluorescence increase is directly proportional to the enzyme activity. Specifications Type Detection/Quantition Usage For quantitative determination of alkaline phosphatase (ALP) activity using stable 4-methylumbelliferyl phosphate substrate. Target Alkaline Phosphatase Detection Fluorometric (Ex/Em 360/450 nm) Supplied Components The following components are supplied with this product. Reagent (pH 10.5) Standard (See Datasheet for specific volumes supplied) Applications Spectrophotometry (fluorometric) Equipment Microplate fluorometer Conditions Shipped Ambient, Store at -20°C, 24 months shelf life.