ALT assay kit LS-K308

for researchALATLDH

Alanine Transaminase (ALT), also known as serum alanine aminotransferase (ALAT) or pyruvic transaminase (SGPT), facilitates the conversion of alanine and a-ketoglutarate to pyruvate and glutamate. ALT plays an important role in gluconeogenesis and amino acid metabolism. ALT is found mainly in the liver, and, to a lesser extent, in kidney, heart, muscle, and pancreas tissues. Normal serum levels of ALT are low, and increased serum ALT activity is widely used as a marker for liver damage. Simple, direct and automation-ready procedures for measuring ALT activity find wide applications in research and drug discovery. This ALT activity assay is based on the quantification of pyruvate produced by ALT. In this assay, pyruvate and NADH are converted to lactate and NAD by the enzyme lactate dehydrogenase (LDH). The decrease in NADH absorbance at 340 nm is proportional to ALT activity. Specifications Type Detection/Quantition Usage For quantitative determination of alanine transaminase activity. Target GPT / Alanine Transaminase Synonyms GPT | AAT1 | ALT1 | Alanine aminotransferase | Alanine aminotransferase 1 | GPT 1 | GPT1 Detection Colorimetric (340 nm Absorbance) Supplied Components The following components are supplied with this product. Assay Buffer LDH Cosubstrate NADH Reagent: Dried (See Datasheet for specific volumes supplied) Applications Spectrophotometry (visible) Equipment Microplate spectrophotometer (visible), Spectrophotometer (visible) Conditions Shipped -20°C Dry Ice, Store at -20°C, 3 months shelf life.