Cholesterol assay kit LS-K304

pharmacologyfor researchNAD

CHOLESTEROL is a sterol and lipid present in the cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones, and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; whereas, low levels (hypocholesterolemia) may be linked to depression, cancer and cerebral hemorrhage. Simple, direct and automation-ready procedures for measuring cholesterol are very desirable. This Cholesterol Assay is based on cholesterol esterase hydrolysis of cholesterol esters to form free cholesterol and cholesterol dehydrogenase catalyzed conversion of cholesterol to cholest-4-ene-3-one, in which NAD is reduced to NADH. The optical density of the formed NADH at 340 nm is directly proportionate to the cholesterol concentration in the sample. Specifications Type Detection/Quantition Usage For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism. Detection Colorimetric (340 nm Absorbance) Supplied Components The following components are supplied with this product. Assay Buffer Enzyme Mix NAD Solution Standard: 300mg/dL Cholesterol Applications Spectrophotometry (visible) Equipment Microplate spectrophotometer (visible) Conditions Shipped -20°C Dry Ice, Store at -20°C, 6 months shelf life.