Ethanol assay kit LS-K299

for researchpharmacologyNAD

Alcoholic drinks are among the most commononly consumed beverages. Studies have shown heavy alcohol consumption may lead to various forms of liver diseases and to increased mortality rates. Quantitative determination of alcohol (ethanol, C2H5OH) has applications in basic research, drug discovery, clinic studies and in the alcoholic industry. Simple, direct and automation-ready procedures for measuring ethanol concentration are very desirable. This ethanol assay kit is based on alcohol dehydrogenase catalyzed oxidation of ethanol, in which the formed NADH is coupled to the formazan (MTT) chromogen. The intensity of the product color, measured at 565 nm, is proportionate to the ethanol concentration in the sample. Specifications Type Detection/Quantition Usage For quantitative determination of ethanol and evaluation of drug effects on alcohol metabolism. Detection Colorimetric (565 nm Absorbance) Supplied Components The following components are supplied with this product. Assay Buffer Enzyme A Enzyme B MTT Solution Enzyme Buffer Stop Reagent NAD Solution Standard (1%) Ethanol (See Datasheet for specific volumes supplied) Applications Spectrophotometry (visible) Equipment Microplate spectrophotometer (visible) Conditions Shipped -20°C Dry Ice, Store at -20°C, 6 months shelf life.