Dilution buffer reagent LS-M20

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IgM-Reducing Assay Diluent is a specially formulated additive to address IgM-mediated conjugate bridging interference in problematic serum and plasma samples tested in antibody sandwich ELISA formats. IgM-Reducing Assay Diluent can be added to standard curve/calibrator and serum/plasma sample wells to help equalize matrix complexity disparity issues. Specifications Large differences in matrix complexity environment between standard curve and sample wells are the main cause for target analyte under-recovery problems in sandwich ELISA analysis. Proprietary additives have been included in IgM-Reducing Assay Diluent to reduce IgM-mediated conjugate bridging interference that can otherwise cause substantial background noise. IgM-Reducing Assay Diluent contains mammalian sera proteins to reduce nonspecific interactions that might occur between the sample matrix proteins and the plate surface. It also contains a proprietary calcium chelating agent designed to inhibit interference from complement and thrombin present in serum and plasma samples. An antimicrobial agent is included in the formulation to allow for bench-top use and storage stability. • pH: 7.4 at 1X • Contains: Mammalian Proteins • Supplied At: 1X