Acid phosphatase reagent 1100005
reagent mediumfor clinical chemistryfor colorimetry

acid phosphatase reagent
acid phosphatase reagent
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Characteristics

Type
reagent medium
Applications
for clinical chemistry, for colorimetry
Tested parameter
acid phosphatase
Method
colorimetric
Storage temperature

Max.: 8 °C
(46 °F)

Min.: 2 °C
(36 °F)

Description

The method is based on the hydrolysis of α-naphtyl phosphate at pH 5.0 by acid phosphatase (ACP) to produce α-naphtol and inorganic phosphate. The pentanediol acts as a phosphate acceptor increasing the reaction sensitivity. The α-naphtol reacts with Fast Red TR, to produce a coloured complex directly proportional to the activity of the ACP in the sample.

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CLINICAL CHEMISTRY

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