Medium reagent 1103005
for colorimetryalkaline phosphatasecolorimetric

Medium reagent - 1103005 - LINEAR CHEMICALS - for colorimetry / alkaline phosphatase / colorimetric
Medium reagent - 1103005 - LINEAR CHEMICALS - for colorimetry / alkaline phosphatase / colorimetric
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Characteristics

Type
reagent medium
Applications
for colorimetry
Tested parameter
alkaline phosphatase
Method
colorimetric
Storage temperature

Min.: 2 °C
(36 °F)

Max.: 8 °C
(46 °F)

Description

Alkaline phosphatase (ALP) catalyze the hydrolysis of 4-nitrophenylphosphate (4-NPP) with the formation of free 4-nitrophenol and inorganic phosphate, acting the alkaline buffer as a phosphate-group acceptor. The reaction is monitored kinetically at 405 nm by the rate of formation of 4-nitrophenol, proportional to the activity of ALP present in the sample.

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