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Medium reagent 1107005for colorimetryfor amylaseenzymatic
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Characteristics
- Type
- reagent medium
- Applications
- for colorimetry
- Tested parameter
- for amylase
- Method
- colorimetric, enzymatic
- Storage temperature
Max.: 8 °C
(46 °F)Min.: 2 °C
(36 °F)
Description
In this direct method α-amylase catalyzes the hydrolysis of 2-chloro-p-nitrophenyl-α-D-maltotrioside (CNP-G3) substrate at pH 6.0 forming 2-chloro-p-nitrophenol (CNP) and free glycosides.
The reaction is monitored kinetically at 405 nm by the rate of formation of the colored CNP produced, proportional to the activity of the α-amylase in the sample.
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