The diarellaBordetella real time PCR Kit is an assay for the detection of DNA of Bordetella pertussis and Bordetella parapertussis in clinical specimens (respiratory samples) using real time PCR microplate systems.
2 Pathogen Information
B. pertussis, B. parapertussis, and B. bronchiseptica are the 3 important human pathogens that belong to the genus Bordetella. B. bronchiseptica can cause infectious bronchitis in dogs and other animals, but rarely infects humans. Only B pertussis produces the pertussis toxin (PT). B. parapertussis has been reported to cause whooping cough either as a single infective agent or with coinfection with B pertussis in almost 40 % of laboratory-confirmed cases. Pertussis (also known as whooping cough or 100-day cough) is a highly contagious bacterial disease. Initially, symptoms are usually similar to those of the common cold with a runny nose, fever, and mild cough. This is then followed by weeks of severe coughing fits. Pertussis is caused by the bacterium Bordetella pertussis.It is an airborne disease which spreads easily through the coughs and sneezes of an infected person. People are infectious to others from the start of symptoms until about three weeks into the coughing fits. Those treated with antibiotics are no longer infectious after five days. Diagnosis is by collecting a sample from the back of the nose and throat. This sample can then be tested by either culture or by polymerase chain reaction.
3 Principle of the Test
The diarellaBordetella real time PCR Kit contains specific primers and duallabeled probes for the amplification and detection of DNA of Bordetella pertussis and Bordetella parapertussis in clinical specimens.