Solution reagent kit virellaNoro

diagnosticfor environmental analysisfor RT-PCR

The virellaNoro real time RT-PCR TM is an assay for the detection of Norovirus RNA (Genogroup I and II) in stool, food and environmental samples using real time PCR microplate systems. 2 Pathogen Information Noroviruses are small non-enveloped RNA viruses belonging to the family of Caliciviridae. They cause approximately 90 % of epidemic non-bacterial outbreaks of gastroenteritis around the world. The viruses are transmitted by fecally contaminated food or water and by person-to-person contact. For this reason, outbreaks of Norovirus infection often occur in closed or semi-closed communities, such as long-term care facilities, hospitals, prisons, dormitories, and cruise ships. Noroviruses are highly contagious and are stable at temperatures between -20°C to +60°C and in acidic environments up to pH 3. Norovirus infections occur throughout the year, however, in Europe, seasonal increases are observed between October and March. The virellaNoro real time RT-PCR Kit TM detects Norovirus strains of high genetic diversity, such as the following: GI: Norwalk, Desert Shield, Winchester, Queensarms, Southhampton, Chiba GII: Lordsdale, Bristol, Melksham, Toronto, Hawaii 3 Principle of the Test The virellaNoro real time RT-PCR Kit TM contains specific primers and duallabeled probes for the amplification and detection of Norovirus RNA (GI and GII) in stool, food and environmental samples after the extraction of RNA from the sample material. The reverse transcription (RT) of viral RNA to cDNA and the subsequent amplification of Norovirus specific fragments are performed in a one-step RTPCR.