Solution reagent kit gastroplexVirus PLUS 2.0

diagnosticfor environmental analysisfor RT-PCR

The gastroplexVirus PLUS 2.0 real time RT-PCR Kit is an assay for the detection of the RNA of Rotavirus, Norovirus GI, Norovirus GII, Sapovirus and Astrovirus and the DNA of Adenovirus in clinical specimens (e.g. stool samples, vomit), environmental and food samples using real time PCR microplate systems. 2 Pathogen Information Acute gastroenteritis is a worldwide major cause of morbidity and mortality. Gastroenteritis or infectious diarrhoea is an inflammation of the gastrointestinal tract. Both the stomach and the small intestine are involved. Typical symptoms are diarrhoea, vomiting, abdominal pain, and cramps, often followed by dehydration. The causative agent can be viral or bacterial. Enteric viruses are the major pathogens for gastroenteritis especially in children. Noro-, Rota-, Adeno-, Sapo- and Astroviruses are the most important viral pathogens. Noroviruses are small non-enveloped RNA viruses belonging to the family of Caliciviridae. They cause approximately 90 % of epidemic non-bacterial outbreaks of gastroenteritis around the world. The viruses are transmitted by fecally contaminated food or water and by person-to-person contact. For this reason, outbreaks of norovirus infection often occur in closed or semi-closed communities, such as long-term care facilities, hospitals, prisons, dormitories, and cruise ships. Noroviruses are highly contagious and are stable at temperatures between -20°C to +60°C and in acidic environments up to pH 3. Norovirus infections occur throughout the year, however, in Europe, seasonal increases are observed between October and March. Infections with Rotavirus are the most common cause of severe diarrhoea among children.