Solution reagent kit virellaEntero 2.0 TM

diagnosticfor RT-PCRliquid

The virellaEntero 2.0 TM real time RT-PCR is an assay for the detection of Enterovirus RNA (Enterovirus, Coxsackievirus A and B, Echovirus, Poliovirus type 1-3) in clinical specimens and environmental samples using open real time PCR systems. 2 Pathogen Information Enteroviruses are highly contagious pathogens belonging to the family of Picornaviridae. They are small, non-enveloped RNA-viruses which are very resistant to environmental conditions. Even at pH 3-9 or in the presence of detergences Enteroviruses remain infectious. The transmission from person to person happens mainly fecal-orally. Contaminated foods and drinking water are important sources of infection. The viruses can be egested in stool even weeks after an acute infection. Infections with Enteroviruses can occur throughout the year, however, in summer, contaminated water in swimming pools or lakes lead to increases in the number of Enterovirus infections. The symptoms caused by Enteroviruses are numerous: infections of the upper respiratory tract, undifferentiated fever, herpangina, hand-foot-mouth-disease, rash disease, paralyses, etc.. 3 Principle of the Test The virellaEntero 2.0 TM real time RT-PCR Kit contains specific primers and hydrolysis probes for the detection of Enterovirus RNA (Enterovirus, Coxsackievirus, Echovirus, Poliovirus) in clinical specimens and environmental samples after the extraction of RNA from the sample material. The reverse transcription (RT) of viral RNA to cDNA and the subsequent amplification of virus specific fragments are performed in a one-step RT-PCR. The amplification can be detected when specific probes are hydrolysed by the Polymerase.