Solution reagent kit virellaTBE

diagnosticfor RT-PCRliquid

The virellaTBE real time RT-PCR TM is an assay for the detection of TBE VirusRNA, extracted from clinical specimens and ticks. 2 Pathogen Information Tick-borne encephalitis (TBE) is a disease caused by the tick-borne encephalitis virus. The disease pattern includes flu-like symptoms and fever. TBE most often manifests as meningitis, encephalitis or meningoencephalitis. However, most patients show no symptoms after infection. The disease is transmitted by the sting of an infected tick, mainly Ixodes ricinus. A causative treatment against TBE is not possible. Beside common precautions like scanning the body for ticks, active vaccination is the most effective method for preventing TBE. Vaccination is recommended for all persons in high-risk areas. Reliable diagnosis can be made on the basis of symptoms, course of disease, anamnesis and serological findings. To better evaluate the risk of infection after the sting of a tick, the tick can be tested by real time RT-PCR for the presence of TBE Virus RNA. There is no curative therapy for TBE. In severe cases interferons are administered. Alltogether the therapy is restricted to symptomatic measures. Bed rest and dimout of the sick room can help to avoid complications. 3 Principle of the Test The virellaTBE real time RT-PCR Kit TM contains specific primers and duallabeled probes for the amplification and detection of TBE Virus RNA, extracted from clinical specimens. The presence of nucleic acid is detected by an increase in fluorescence due to hydrolysis of the probes during amplification. The fluorescence of the pathogen-specific probes is measured in the FAM channel.