The diarellaBorrelia real time PCR TM is an assay for the detection of Borrelia burgdorferi sensu lato DNA, extracted from clinical specimens and ticks.
2 Pathogen Information
Borrelia are gram-negative bacteria of the spirochaete family. Members of the genus Borrelia are the causative agents of two important tick-borne diseases: relapsing fever and Lyme disease.
In Europe, Lyme borreliosis is the most common vector-borne disease. The highest incidence is reported from Austria, Switzerland, the Czech Republic, Germany, Slowenia, as well as from the northern countries bordering the Baltic Sea.
Lyme Borreliosis is a multi-system disorder, which can lead to severe complications of the neurological system, the heart and the joints. At an early stage of its manifestation borreliosis is treatable with antibiotics, however, clinical diagnosis is complicated. Antibodies are not detectable in the blood until weeks after infection and symptoms are highly variable.
Analysis of ticks offers the possibility to identify the risk of infection very quickly, and therefore minimising the delay of an antibiotics treatment.
3 Principle of the Test
The diarellaBorrelia real time PCR Kit TM contains specific primers and duallabeled probes for the amplification and detection of Borrelia burgdorferi sensu lato DNA, extracted from clinical specimens and ticks.