Solution reagent kit diarellaMRSA 3.0 TM

diagnosticfor RT-PCRliquid

The diarellaMRSA 3.0 TM real time PCR is an assay for the detection of the DNA of MRSA in clinical specimens. 2 Pathogen Information Staphylococcus aureus are gram-positive coccal bacteria which are ubiquitously found in the environment. About 25-30 % of the human population are long-term carriers of S. aureus because the bacteria are frequently part of the skin flora found in the nose and on skin. S. aureus can cause a range of illnesses such as minor skin infections, like furuncles and abscesses, pyomyositis, but also life-threatening diseases such as pneumonia, endocarditis, toxic shock syndrome (TSS), and sepsis. Of increasing importance worldwide are Methicillin-resistant Staphylococcus aureus (MRSA) strains. Especially in hospitals MRSA present a danger, because they are resistant to all ß-lactam antibiotics (e.g. penicillin) and often possess further resistances to other anitbiotics. 3 Principle of the Test The diarellaMRSA 3.0 TM real time PCR contains specific primers and duallabeled probes for the amplification and detection of MRSA DNA in clinical specimens. The PCR targets the orfX/SSCmec junction and allows for the detection of MRSA in clinical samples, even those containing CoagulaseNegative Staphylococci. Furthermore, diarellaMRSA 3.0 real time PCR Kit allows the detection of the methicillin resistance gene mecA/mecC, to eliminate false positive results through dropout mutants. The presence of nucleic acid is detected by an increase in fluorescence due to hydrolysis of the probes during amplification. The fluorescence of the pathogen-specific probes is measured in the FAM channel.