Solution reagent kit virellaPRRSV

diagnosticfor RT-PCRliquid

The virellaPRRSV real time RT-PCR Kit is an assay for the simultaneous detection of the RNA of the North American (NA), European (EU), and the highly pathogenic (HP) strain of the NA genotype of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Extracted RNA from swine blood, serum, tissue, bronchial swabs, saliva, bronchial lavage, semen, and cell culture supernatant samples can be used for analysis. 2 Pathogen Information PRRSV infections are highly prevalent in swine and lead to high economical losses. The panzootic disease causes reproductive failure in breeding stock and respiratory tract illness in young pigs. PRRSV is a member of the genus Arterivirus, family Arteriviridae, order Nidovirales. The two prototype strains of PRRSV are the North American strain, VR-2332, and the European strain, the Lelystad virus (LV). The European and North American PRRSV strains cause similar clinical symptoms, but represent two distinct viral genotypes whose genomes diverge by approximately 40 %. In the early 2000s a highly pathogenic strain of the North American genotype emerged in China. This strain, HP-PRRSV, is more virulent than all other strains, and causes great losses in Asian countries. 3 Principle of the Test The virellaPRRSV real time RT-PCR contains specific primers and hydrolysis probes for the detection of RNA of the NA, EU, and HP strains of PRRSV. Extracted RNA from swine blood, serum, tissue, bronchial swabs, saliva, bronchial lavage, semen, and cell culture supernatant samples can be used for analysis. The reverse transcription (RT) of viral RNA to cDNA