Solution reagent kit respiPig 1

veterinary laboratorydiagnosticfor real-time PCR

The respiPig 1 real time RT-PCR Kit is an assay for the simultaneous detection of the RNA of the North American (NA), European (EU) genotypes of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), the RNA of type A influenza viruses (Flu A) and the DNA of Mycoplasma hyopneumoniae (M. hyopneumoniae). Extracted RNA and DNA from swine blood, serum, tissue, bronchial swabs, saliva, bronchial lavage, semen, and cell culture supernatant samples can be used for analysis. 2 Pathogen Information PRRSV infections are highly prevalent in swine and lead to high economical losses. The panzootic disease causes reproductive failure in breeding stock and respiratory tract illness in young pigs. PRRSV is a member of the genus Arterivirus, family Arteriviridae, order Nidovirales. The two prototype strains of PRRSV are the North American strain, VR-2332, and the European strain, the Lelystad virus (LV). The European and North American PRRSV strains cause similar clinical symptoms, but represent two distinct viral genotypes whose genomes diverge by approximately 40 %. Swine influenza is a respiratory disease of pigs caused by type A influenza viruses that regularly cause outbreaks of influenza in pigs. Like human influenza viruses, there are different subtypes and strains of swine influenza viruses. The main swine influenza viruses circulating in U.S. pigs in recent years have been, swine triple reassortant (tr) H1N1 influenzavirus, trH3N2 virus, and trH1N2. The incubation period is short, less than 48 hours. The onset can be extremely rapid and dramatic.