Solution reagent kit NukEx Mag

for DNA extractionfor RNA extraction

The NukEx Mag RNA/DNA Kit is designed for rapid manual and automated purification of nucleic acids from a wide range of samples (e.g. human samples, veterinary samples, insects, food samples, etc.). The kit is designed for use with automated magnetic processors (e.g. KingFisher™ Flex or Duo Prime, Microlab STAR, Freedom EVO, Biocomma M32, M96). Each IVD application of this sample preparation procedure in combination with an in vitro diagnostic downstream nucleic acid test must be evaluated for the respective IVD parameter. 2 Mode of Action a) Samples are lysed by incubation in Working Solution (Binding Buffer (P1) substituted with 2-propanol). Nucleic acids are bound to the Magnetic Beads. b) Bound nucleic acids are washed with Inhibitor Removal Buffer (P2) in order to remove PCR inhibitors from the sample. c) Bound nucleic acids are washed with Wash Buffer (P3) in order to purify them from salts, proteins and other cellular impurities. d) Purified nucleic acids are eluted from the Magnetic Beads with Elution Buffer (P4). Purified nucleic acids can be used directly for downstream applications. Purified nucleic acids can be used directly for downstream applications. the customer needs to decide, which consumables are necessary for the extraction process. ▪ Laboratory equipment according to national safety instructions ▪ Nuclease-free 1.5 or 2.0 ml microcentrifuge tubes ▪ Separation plate for magnetic beads separation, e.g. Square-well Block (96-well block with 2.1 ml square-wells) ▪ Elution plate for collecting purified nucleic acids