Solution reagent kit NukEx Mag Extreme SC

sample preparationfor DNA extractiontissue

The NukEx Mag Extreme SC and NukEx Mag Extreme SL Kits are designed for rapid manual and automated extraction of nucleic acids from a wide range of difficult samples (e.g. human samples, veterinary samples, insects, food samples, etc.). The kits are designed for use with tissue homogenizers for mechanical disruption of samples and subsequent purification of nucleic acids using magnetic beads. The purified nucleic acids can be used directly as template for real time (Reverse Transcription-) PCR or any kind of enzymatic reactions. NukEx Mag Extreme Kits allow easy automation on common liquid handling instruments or automated magnetic separators. 2 Mode of Action a) Samples are lysed by mechanical disruption in the presence of Extraction Buffer 1 (EX1) and subsequent adding of Extraction Buffer 2 (EX2). b) Nucleic acids are bound to the NukEx Magnetic Beads (MB). c) Bound nucleic acids are washed with Inhibitor Removal Buffer (P2) in order to remove PCR inhibitors from the sample. d) Bound nucleic acids are washed with Wash Buffer (P3) in order to purify them from salts, proteins and other cellular impurities. e) Purified nucleic acids are eluted from the NukEx Magnetic Beads with Elution Buffer (P4). 4 Equipment and Reagents to be supplied by User Note: consumables not included in the kit are dependent of the mode of sample preparation, e.g. manual extraction or extraction using magnetic particle processors such as KingFisher Flex. ▪ Homogenizer (Fast Prep, Precellys, TissueLyser or equivalent) ▪ Nuclease-free 1.5 or 2.0 ml microcentrifuge tube