Solution reagent kit NukEx PLUS

for nucleic acidsfor saliva samplesliquid

The NukEx PLUS Nucleic Acid Release Reagent is designed for the release of DNA from bacterial cultures and swabs. The crude lysates can be directly applied in molecular assays, e.g. real time PCR. 2 Principle of the Test The NukEx PLUS Nucleic Acid Release Reagent causes the lysis of bacteria. The lysis is performed for 15 minutes at room temperature. Nucleic acids released with NukEx PLUS Nucleic Acid Release Reagent can be analysed by employing the supernatants obtained directly in the subsequent molecular assay. Pooling of the lysates prior to analysis is possible; however, it is subject to the purpose and regulations of the particular application. 3 Package Contents 4x 8.75 ml NukEx PLUS Nucleic Acid Release Reagent sufficient for 100 reactions. 4 Equipment and Reagents to be supplied by User ▪ Laboratory equipment according to national safety instructions ▪ Sterile pipet tips with filter ▪ Nuclease-free 1.5 or 2.0 ml microcentrifuge tube ▪ Optional: Liquid handling systems for automation (e.g. Myra, Bio Molecular Systems) 5 Transport and Storage The NukEx PLUS Nucleic Acid Release Reagent must be stored ≤ -18°C. If properly stored, the product is stable until the date of expiry printed on the label. Please note, that improper storage will adversely impact nucleic acid release due to decreased enzymatic activity. Therefore, NukEx PLUS Nucleic Acid Release Reagent is always shipped on dry ice. 6 Important Notes ▪ The NukEx PLUS Nucleic Acid Release Reagent must be utilized by qualified personnel only. ▪ Good Laboratory Practice (GLP) has to be applied. ▪ Clinical samples must always be regarded as potentially infectious material