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Solution reagent kit KR10082
diagnosticliquidDPD

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Characteristics

Type

solution

Applications

diagnostic

Format

liquid

Tested parameter

DPD, bilirubin

Method

colorimetric

Storage temperature

Max.: 8 °C
(46 °F)

Min.: 2 °C
(36 °F)

Description

Direct bilirubin (conjugated) reacts with the diazonium salt 2,4-dichlorophenyldiazonium (2,4-DPD) in the presence of sulfamic acid to form azo-bilirubin, this coloured complex can be measured photometrically at 546 nm. Of the two bilirubin fractions in serum –bilirubin-glucuronide (conjugated) and free bilirubin which is bound to albumin (unconjugated)– only the first reacts directly, while free bilirubin reacts after being displaced from protein (albumin) by an accelerator. The difference between the two measurements: total bilirubin (with accelerator) and direct bilirubin (without accelerator) enables to calculate indirect bilirubin. The terms «direct» and «indirect» bilirubin refers exclusively to the reaction characteristics in the presence or absence of an accelerator or solubilizer and are only approximate equivalents of the two bilirubin fractions.

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Solution reagent kit KR10082diagnosticliquidDPD

Characteristics

Description

Catalogs

Other LINEAR CHEMICALS products

CLINICAL CHEMISTRY

Solution reagent kit KR10082
diagnosticliquidDPD