Solution reagent kit 1119005
diagnosticliquidfor cholinesterase

solution reagent kit
solution reagent kit
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Characteristics

Type
solution
Applications
diagnostic
Format
liquid
Tested parameter
for cholinesterase
Method
enzymatic, colorimetric
Storage temperature

Max.: 8 °C
(46 °F)

Min.: 2 °C
(36 °F)

Description

Cholinesterase (CHE) catalyzes the hydrolysis of butyrylthiocholine substrate forming butyrate and thiocholine. The latter reduces 5,5´- mercaptobis-2-nitrobenzoic acid (DMNB) to 5-mercapto-2- nitrobenzoate (5-MNBA), a colored compound. The reaction is monitored kinetically at 405 nm by the rate of formation of the yellow color produced, proportional to the activity of CHE in the sample.

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