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Solution reagent kit KR10150
diagnosticliquidcreatinine

Solution reagent kit - KR10150 - LINEAR CHEMICALS - diagnostic / liquid / creatinine

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Characteristics

Type

solution

Applications

diagnostic

Format

liquid

Tested parameter

hydrogen peroxide, creatinine

Method

enzymatic, colorimetric

Storage temperature

Max.: 8 °C
(46 °F)

Min.: 2 °C
(36 °F)

Description

This procedure is based upon an improved enzymatic method originally devised to evaluate creatinine in serum and urine. The test is performed in two steps. In the first step creatine is removed during the first minutes of the preincubation stage of the sample with creatinase. In the second step the addition of creatininase, acting also as the reaction starter, hydrolyzes the creatinine of the sample in the presence of sarcosine oxidase (Sar OD) with production of hydrogen peroxide. Creatinine + H2O Creatine Sarcosine + H2O + O2 H2O2 + Glicine + HCHO The hydrogen peroxide produced from the oxidase reaction is cuantified by a Trinder’s type reaction in which the chromogenic derivative HTIB and 4- aminoantipyrine (4-AA) are condensed in the presence of peroxidase (POD) to form a red quinoneimine dye. 4-AA + HTIB Red quinoneimine + H2O The rate of color development is proportional to the concentration of creatinine in the sample.

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Solution reagent kit KR10150 diagnosticliquidcreatinine

Characteristics

Description

Catalogs

Other LINEAR CHEMICALS products

CLINICAL CHEMISTRY

Solution reagent kit KR10150
diagnosticliquidcreatinine