Solution reagent kit DIRECT
diagnosticliquidcholesterol

solution reagent kit
solution reagent kit
solution reagent kit
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Characteristics

Type
solution
Applications
diagnostic
Format
liquid
Tested parameter
lipoprotein, for LDL, cholesterol
Method
enzymatic, colorimetric
Storage temperature

Max.: 8 °C
(46 °F)

Min.: 2 °C
(36 °F)

Description

The assay is based on a modified polyvinyl sulfonic acid (PVS) and polyethyleneglycolmethylether (PEGME) coupled classic precipitation method with the improvements in using optimized quantities of PVS/PEGME and selected detergents. LDL, VLDL, and chylomicron (CM) react with PVS and PEGME and the reaction results in inaccessibility of LDL, VLDL and CM by cholesterol oxidase (CHOD) and cholesterol esterase (CHER), whereas HDL reacts with the enzymes. Addition of R2 containing a specific detergent releases LDL from the PVS/PEGME complex. The released LDL reacts with the enzymes to produce H2O2 which is quantified by the Trinder reaction. The intensity of the color formed is proportional to the LDLc concentration in the sample.

Catalogs

NEOS qPCR
NEOS qPCR
2 Pages

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