Solution reagent kit 1143010
diagnosticliquidlipase

solution reagent kit
solution reagent kit
solution reagent kit
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Characteristics

Type
solution
Applications
diagnostic
Format
liquid
Tested parameter
lipase
Method
enzymatic, colorimetric
Storage temperature

Max.: 8 °C
(46 °F)

Min.: 2 °C
(36 °F)

Description

The method for the determination of lipase is based on the cleavage of specific chromogenic lipase substrate 1,2-Odilaurylrac- glycero-3-glutaric acid-(6’methyl-resorufin)-ester emulsified in stabilized micro-particles. In the presence of specific activators of pancreatic lipase as colipase, calcium ions and bile acids, the substrate is con-verted to 1,2-O-dilauryl-rac-glycerol and glutaric acid-6’-methylresorufinester which decomposes spontaneously to glutaric acid and methylresorufin. The increase of absorbance at 580 nm, due to methylresorufin formation, is proportional to the activity of lipase in the sample.

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