Qualitative determination of the D antigen on human red blood cells.
SLIDE AND TUBE TESTS
PRINCIPLE
The presence of the D antigen is determined by testing the test blood cells against the antibody with a known anti-D specificity.
The reagent will cause direct agglutination of test red cells that carry the D antigen and indirect agglutination of test red cells that are Category DVI in the antiglobulin (AHG) phase of testing.
The agglutination of the red blood cells being tested indicates the presence in them of the corresponding antigen. No agglutination generally indicates the absence of the D antigen.