Solution reagent kit 6107310

diagnostichCGβ-hCG

Human chorionic gonadotropin (hCG) is a glycoprotein hormone normally produced by the placenta during pregnancy. The hCG molecule consists of two combined, dissimilar subunits designated alpha and beta. The beta subunit, with a molecular weight of approximately 30,000 daltons, confers biological and immunological specificity to the entire hCG molecule by virtue of its unique amino acid sequence and content. The alpha subunit, with a molecular weight of approximately 18,000 daltons, is essentially identical to the alpha subunit of the pituitary glycoprotein hormones: luteinizing hormone (LH), follicle-stimulating hormone (FSH), and thyroidstimulating hormone (TSH). The hCG Quantitative Test is based on a solid phase enzymelinked immunosorbent assays (ELISA). The assay system utilizes a mouse monoclonal anti--hCG antibody for solid phase (microtiter wells) immobilization and a mouse monoclonal anti-β-hCG antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test specimen (serum) is added to the -hCG antibody coated microtiter wells and incubated with the Zero Buffer at room temperature for 30 minutes. If hCG is present in the specimen, it will combine with the antibody on the wells. The wells are then washed to remove any residual test specimen, and β-hCG monoclonal antibody labeled with horseradish peroxidase (conjugate) is added. The conjugate will bind immunologically to the hCG on the wells, resulting in the hCG molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation at room temperature for 15 minutes, the wells are washed with water to remove unbound-labeled antibodies.