Antifungal reagent disk

clinicalfungi

Antifungal Disc are paper discs with special features that are impregnate with antimicotics, used for the susceptibility test of yeasts according to the CLSI method. Each antimicotic is available in packages of 50 and 250 discs. CONTENTS OF THE PACKAGES Discs in cartridge The 50-test package contains 1 cartridge with 50 discs packaged in blister with silica gel. The 250-test package contains 5 cartridges with 50 discs, packaged in blister with silica gel. Each package also contains a transparent resealable bag. Discs in canister The canister contains 250 discs and a desiccant tablet. METHOD PRINCIPLE The discs are applied to the surface of a culture medium inoculated with the suspension containing pure colonies of the microorganism under examination. After incubation, the plates are examined, the inhibition halos around each disc are examined and compared with the standard inhibition haloes: in this way the microorganisms are defined as being susceptible, susceptible-dose dependent, resistant or non susceptible to the tested antimicrobial agents. COMPOSITION The discs are made of high-quality paper in compliance with WHO and FDA specifications. Each disc is impregnated with a standardised amount of antimicotic. GATHERING AND KEEPING SAMPLES The colonies that are to be subjected to the susceptibility test are taken up by culture media that have been previously swabbed with the sample under examination. In the case of mixed colonies the strains must be purified in blood medium or Sabouraud Dextrose Agar before they are swabbed on the plates for the susceptibility test.