HCG test kit

pregnancyserumenzyme immunoassay

hCG EIA Test Kit An enzyme immunoassay (EIA) for the quantitative detection of hCG (human chorionic gonadotropin) in human serum. The hCG EIA Test Kit is an enzyme immunoassay for in vitro quantitative determination of hCG level in human serum. It is intended as an aid in the early detection of pregnancy. Human chorionic gonadotropin (hCG) is a sialoglycoprotein with a molecular weight of approximately 46,000 Daltons 1. The hCG molecule consists of two combined, dissimilar subunits designated alpha and beta. The beta subunit, with a molecular weight of approximately 30,000 Daltons, confers biological and immunological specificity to the entire hCG molecular by virtue of its unique amino acid sequence and content. The alpha subunit, with a molecular weight of approximately 18,000 Daltons, is essentially identical to the alpha subunit of the pituitary glycoprotein hormones; LH, FSH and TSH. The measurement of hCG, by assay systems with suitable sensitivity and specificity has proven great value in the detection of pregnancy and the diagnosis of early pregnancy disorders. According to the literature, hCG is detectable as early as 10 days after ovulation, reaching 100 mIU/mL by the first missed period. At the time for the next ovulation, the hCG level is 200 mIU/mL (approximately 28 days after conception) 2. A peak of 50,000 or even 100,000 mIU/mL is attained by the third month, then a gradual decline is observed 3. With the availability of sensitive quantitative assays for the measurement of serum hCG, it has been shown that hCG levels can be useful in prediction of spontaneous abortions, aiding in the detection of ectopic pregnancy and multiple gestation.