Stain reagent F23003

for cell countingliquid

Propidium iodide is a cell-permeable stain that binds to nucleic acids. Nonviable cells and cells with damaged membranes are the only ones that can take up propidium iodide, since intact membranes cannot penetrate it. Once bound to nucleic acids, its fluorescence is multiplied by 20-30, causing the cell to fluoresce red. LUNA™ Automated Fluorescence Cell Counters can use propidium iodide stain (F23002) with acridine orange stain (F23002) to assess cell viability. Viable nucleated cells will fluoresce green and non-viable cells will fluoresce red. Due to Förster resonance energy transfer (FRET), the signal from propidium iodide absorbs the signal from acridine orange in non-viable cells, ensuring that double-positive results do not occur. Specifications Molecular formula - C27H34I2N4 Molecular weight - 668.39 g/mol Cell permeability - Membrane permeant Excitation/emission - 493/636 nm (in aqueous solution) 533/617 nm (when bound to nucleic acids)