Clinical chemistry assay kit CC00150citric acidurineenzymatic
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Characteristics
- Application field
- for clinical chemistry
- Tested parameter
- citric acid
- Sample type
- urine
- Analysis mode
- enzymatic, colorimetric
Description
Clinical Chemistry Specialties
/ Citric Acid in Urine
Enzymatic colorimetric determination of citric acid in urine.
The Citric Acid (citrate) is changed in oxalacetate and acetate by CL (Citrate lyase).
In presence of Malate-dehydrogenase (MDH) and Lactate-dehydrogenase (LDH),
the oxalacetate and pyruvate (decarboxilated product of oxalacetate), are trasformed in
L-Malate and L-Lactate, giving oxidization of NADH in NAD+.
The formation of NAD+ causes a diminution of absorbance at 340 n
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