Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.
Components
Capture mAb - MTH21
Detection mAb - MTH29, biotin
Enzyme conjugate - Streptavidin-HRP
Intended use
This ELISpot Flex kit is intended for the enumeration of cells secreting hamster IFN-γ using the ELISpot assay. For research use only. Not for use in diagnostic procedures.
Product details
Application - ELISpot
Analyte - IFN-γ
Reactivity - Hamster
Specificity - This kit is based on a matched pair of mAbs specific for hamster IFN-γ.
Performance
Hamster IFN-γ ELISpot (200,000 splenocytes).
Analyte information
IFN-γ
Analyte description
Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.
Alternative names - Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg
Cell type - T cell, Tc, Th1, NK cell