Research assay kit FSP-013602-P12-1

infectionvirusSARS-COV-2

Paves your way to functional analysis of a specific research question. Intended use This FluoroSpot Path kit is intended for the enumeration of cells secreting human IFN-γ, granzyme B, and IL-2 in response to SARS-CoV-2 (S+NMO) peptides using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures. Peptide pool composition The SARS-CoV-2 S defined peptide pool contains 100 peptides from the human SARS-CoV-2 virus. The peptides are derived from the spike (S) protein. The SARS-CoV-2 NMO defined peptide pool contains 101 peptides from the human SARS-CoV-2 virus. The peptides are derived from the nucleoprotein (N), membrane protein (M), and open reading frame (ORF) proteins (O): ORF1, nsp3, ORF-3a, ORF-7a, and ORF8. Performance Human IFN-γ/Granzyme B/IL-2 FluoroSpot. PBMC were prepared from an individual after receiving two doses of SARS-CoV-2 vaccine and after subsequent SARS-CoV-2 infection. PBMC were incubated 48 h in the absence or presence of the SARS-CoV-2 S defined peptide pool or NMO defined peptide pool. All cells were co-stimulated with anti-CD28 mAb. Single cytokine secreting cells as well as double- and triple-secreting cells were determined. Analysis was done with using Mabtech IRIS. Analyte description - Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells.Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype.