Research assay kit FSP-0102-P2-1

for CMVimmunoassay

Paves your way to functional analysis of a specific research question. Intended use This FluoroSpot Path kit is intended for the enumeration of cells secreting human IFN-γ and IL-2 in response to CMV peptides using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures. Peptide pool composition The CMV peptide pool contains 42 peptides derived from CMV. The antigens represented in the peptide pool are pp50, pp65, IE1, IE2, and Envelope glycoprotein B. Of the included peptides, 28 are MHC class I restricted and 14 are MHC class II restricted. Performance Human PBMC (250,000 cells/well) were incubated overnight in the absence or presence of the CMV peptide pool for human CD4 and CD8 T cells (Mabtech) and the number of cells secreting IFN-γ and/or IL-2 was analyzed by FluoroSpot. Single cytokine secreting cells as well as double-secreting cells were determined. Spot center was used to identify spots from double-secreting cells, visualized in an image overlay of LED490, FITC (IFN-γ) and LED550,Cy3 (IL-2). Spot analysis was made with Mabtech IRIS. IFN-γ Analyte description Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens. Alternative names - Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg, IFG, IFI, IMD69