Monoclonal antibody IL4-3

for researchfor flow cytometryfor ELISA

This monoclonal antibody enables specific detection of human IL-4-producing cells in flow cytometry. Recommendation 5 µl is recommended for staining of 1 million cells in a total volume of 50 µl. PF647P is excited by the red laser (633 nm). The excitation max is 654 nm and the emission max is 672 nm. Product details Product - Anti-human IL-4 mAb (IL4-3), PF647P Application - Flow cytometry Analyte - IL-4 Conjugate - PF647P Clonality - Monoclonal Immunogen - Recombinant human IL-4 Host - Rat Reactivity - Human, Non-human primates Specificity - Native and recombinant human IL-4 Cross-reactivity - Cross-reacts with IL-4 from rhesus and cynomolgus macaques. For more information please check the Cross-reactivity guide section below. Supplied in - PBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm). Performance Detection of IL-4 by flow cytometry in viable cynomolgus lymphocytes. Non-stimulated (left panel) and stimulated (PMA/ionomycin; right panel), fixed and permeabilized cells were stained with anti-human IL-4 mAb IL4-3-PF4647P in combination with anti-CD3-PE. Flow cytometry was performed on a BD FACSVerse system. Detection of IL-4 by flow cytometry in viable human lymphocytes. Non-stimulated (left panel) and stimulated (PMA/ionomycin; right panel), fixed and permeabilized cells were stained with anti-human mAb IL4-3-PF647P in combination with anti-CD3-BV421. Flow cytometry was performed on a BD FACSVerse system. Analyte description Interleukin 4 (IL-4) is produced primarily by Th2 cells, mast cells, eosinophils, and basophils. IL-4 was first identified to costimulate B cell growth