Research test kit 3560-1HP-DP-1

rheumatoid factorserumplasma

Optimized to block rheumatoid factor and therefore suitable when studying samples from patients with autoimmune disease. Intended use This ELISA PathRF kit is intended for the quantification of human IL-8 (CXCL8) in serum and plasma containing rheumatoid factor (RF). For research use only. Not for use in diagnostic procedures. ELISA PathRF kits ELISA PathRF kits are designed to eliminate potential interference from RF and heterophilic antibodies. The combination of a recombinant detection mAb and RF-block diluent prevents RF and heterophilic antibodies from cross-linking the assay antibodies, effectively reducing the risk of false-positive signal. RF and heterophilic antibodies can be found at high concentrations in serum and plasma from individuals with autoimmune diseases such as rheumatoid arthritis. The kit has been validated with RF-containing plasma. Shipping Shipped at ambient temperature. Storage Store detection mAb, Streptavidin-HRP, buffers/solutions, and substrate at 4-8 °C upon receipt. The ELISA standard should be stored at -20 °C. Plates may be kept at room temperature. Shelf life At least 12 months from date of receipt. Sensitivity 2 pg/ml The lowest concentration that can be detected, but not necessarily quantified with precision and accuracy. This was determined by adding 2 standard deviations to the mean OD of background wells. IL-8 (CXCL8) Analyte description Interleukin 8 (IL-8) is a proinflammatory cytokine produced by different types of cells, including monocytes, macrophages, and endothelial cells. Inflammatory stimuli, such as LPS, strongly induce IL-8 production by monocytes.