Research test kit 3113-2A

for caninesALPELISA

Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice. Components Capture mAb - MT13 Detection mAb - MT166, biotin Enzyme conjugate - Streptavidin-ALP Intended use This ELISpot Flex kit is intended for the enumeration of cells secreting canine IFN-γ using the ELISpot assay. For research use only. Not for use in diagnostic procedures. Product details Product - ELISpot Flex: Canine IFN-γ (ALP) Application - ELISpot Analyte - IFN-γ Reactivity - Dog Specificity This kit is based on a matched pair of mAbs enabling specific detection of canine (dog) IFN-γ. Cross-reactivity The mAbs react with horse IFN-γ and cross-react with IFN-γ from dog. For more information please check the Cross-reactivity guide section below. Performance PBMC from dog were incubated in the presence (25,000 cells/well) or absence (200,000 cells/well) of Phytohaemagglutinin (PHA) and the number of cells secreting IFN-γ was analyzed by canine IFN-γ ELISpot. Veterinary reagents Species reactivities of our veterinary assays and included monoclonal antibodies are summarized in the tables below. Symbol keys: Cross-reactivity Verified by Mabtech and/or by others. Tests performed in ELISpot and/or ELISA. Indications of Cross-reactivity Based on reports from others or by analysis using recombinant proteins. Blank boxes Represent cross-reactivities not evaluated. Analyte description - Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells.