Research test kit 3125-2A

for poultryALPcell

Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice. Components Capture mAb - MT6C2 Detection mAb - MT7C10, biotin Enzyme conjugate - Streptavidin-ALP Intended use This ELISpot Flex kit is intended for the enumeration of cells secreting chicken IFN-γ using the ELISpot assay. For research use only. Not for use in diagnostic procedures. Product details Product - ELISpot Flex: Chicken IFN-γ (ALP) Application - ELISpot Analyte - IFN-γ Reactivity - Chicken Specificity - This kit is based on a matched pair of mAbs specific for chicken IFN-γ. Performance Chicken PBMC (500,000 cells/well) were incubated overnight in the presence PMA/ionomycin and the number of cells secreting IFN-γ was analyzed by ELISpot. Analyte information IFN-γ Analyte description Interferon-γ (IFN-γ) is the only type II interferon. This proinflammatory cytokine is secreted by activated T cells and NK cells. It activates macrophages and endothelial cells and regulates immune responses by affecting APCs, T cells, and B cells. Production of IFN-γ by helper T cells and cytotoxic T cells is a hallmark of the Th1-type phenotype. Thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens. Alternative names - Interferon-γ, Interferon-gamma, IFN-γ, IFN-gamma, IFN-g, IFNg Cell type - T cell, Tc, Th1, NK cell