Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.
Components
Capture mAb - MT24/JC5-1
Detection mAb - MTG2c, biotin
Enzyme conjugate - Streptavidin-HRP
Stimuli - R848 - Recombinant mouse IL-2
Intended use
This ELISpot Flex kit is intended for the enumeration of cells secreting mouse IgG2c using the ELISpot assay. For research use only. Not for use in diagnostic procedures.
With this kit, detection of antigen-specific IgG2c secreting B cells can be performed using coated antigen. If detection with biotinylated antigen is preferred, the plate should instead be coated with the subclass specific antibody.
The IgG2c allele is expressed in certain inbred mouse strains e.g., C57BL/6, C57BL/10, SJL and NOD.
IgG2c
Analyte description -
IgG (Immunoglobulin G) is the most abundant Ig isotype in serum, making up approximately 80% of all serum Immunoglobulins. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG2c allele is expressed (instead of IgG2a) in certain inbred mouse strains, e.g., C57BL/6, C57BL/10, SJL, and NOD.
Alternative names - Immunoglobulin G2c, IgG2c
Cell type - B cell