Monoclonal antibody 97B4

for researchfor flow cytometryIgG

This monoclonal antibody is recommended as mouse IgG2a isotype control in flow cytometry. It has been used as isotype control with cells from different species. Recommendation 5 µl is recommended for staining of 1 million cells in a total volume of 50 µl. PF647P is excited by the red laser (633 nm). The excitation max is 654 nm and the emission max is 672 nm. Product details Analyte - Non-specific Conjugate - PF488P Immunogen - Bacterial antigen Host - Mouse Specificity - Bacterial protein Supplied in - PBS with 0.2% BSA and 0.09% sodium azide. Sterile-filtered (0.2 µm). Analyte description - Isotype controls are often used as negative controls in flow cytometry, immunochemistry, or Western blot experiments. An isotype control is an antibody that doesn't bind the target analyte but otherwise matches the properties of the primary antibody. For example, an isotype control should be conjugated in the same way and match the isotype of the target-specific antibody used in the assay.