Research test kit 3192-2H

veterinaryfor sheepcell

Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice. Components Capture mAb - MT52B34 Detection mAb - MT11B37, biotin Enzyme conjugate - Streptavidin-HRP Intended use This ELISpot Flex kit is intended for the enumeration of cells secreting sheep IL-5 using the ELISpot assay. For research use only. Not for use in diagnostic procedures. Product details Application - ELISpot Analyte - IL-5 Reactivity - Sheep Specificity - This kit is based on a matched pair of mAbs enabling specific detection of sheep IL-5. Cross-reactivity - The mAbs react with bovine IL-5 and cross-react with IL-5 from sheep. For more information please check the Cross-reactivity guide section below. Performance Sheep PBMC were incubated for 40 h in the absence (200,000 cells/well) or presence (100,000 cells/well) of PMA/ionomycin and the number of cells secreting IL-5 was analyzed by ELISpot. Species reactivities of our veterinary assays and included monoclonal antibodies are summarized in the tables below. Symbol keys: Cross-reactivity Verified by Mabtech and/or by others. Tests performed in ELISpot and/or ELISA. IL-5 Analyte description Interleukin 5 (IL-5) is a pleiotropic cytokine primarily produced by T and NK cells. IL-5 specifically controls the survival, differentiation, and chemotaxis of eosinophils. But initially, IL-5 was characterized by its ability to support the growth and terminal differentiation of B cells. Alternative names - Interleukin-5, IL-5, IL5, BCGF-2, TRF Cell type - T cell, Th2, NK cell