Isolating DNA from tissues and/or cells is a fundamental step in many molecular biology and genetic research techniques.
Isolating DNA from tissues and/or cells is a fundamental step in many molecular biology and genetic research techniques. It serves several purposes and enables scientists to study and manipulate DNA for various applications.
This kit is intended for manual and automated isolation of genomic DNA from mammalian tissue samples and eukaryotic cells. Processing time for the preparation of 96 samples is about 40 minutes plus an additional pre-lysis incubation of 15 min (for cells), 1-3 h or overnight for tissue samples.
Safety
The kit does not require phenol/chloroform extraction or alcohol precipitation and eliminates the need for repeated centrifugation, vacuum filtration or column separation. It allows safe handling of potentially infectious samples, and is designed to avoid sample-to-sample cross-contaminations. The obtained DNA can be used directly for downstream applications such as PCR, or any kind of enzymatic reaction.
Features
Short and easy protocols, 96 samples in 40 minutes
Avoid dangerous substances like phenol/chloroform
Eliminates need for repeated centrifugation, vacuum or column separations
Consistently high yield and purity of genomic DNA
Excellent integrity DIN > 8.1
Suitable for many downstream applications
Easy to automate
Minimal accessory requirements
PurePrep / KingFisherTM / Biosprint 96 / MagMaxTM protocols and consumables available
Compatible with general liquid handling robots (e.g. Hamilton®, TECAN®)
Magnetic separators for microtubes and microplates separately available