A Liquid Biopsy is a non-invasive medical test that involves analyzing a sample of body fluids, typically blood, to obtain information about a person’s health status. Unlike traditional biopsies, which involve extracting tissue samples through surgical procedures, liquid biopsies provide valuable insights into various aspects of health and disease through the analysis of molecules present in the bodily fluids.
This kit is intended for Liquid Biopsy, the (automated) purification of circulating cell-free DNA from human plasma, serum or urine samples. Processing time for the preparation of 24 samples is about 60 minutes.
Safety
The kit requires no phenol/chloroform extraction or alcohol precipitation and eliminates the need for repeated centrifugation, vacuum filtration or column separation. It allows safe handling of potentially infectious samples. The obtained cfDNA can be used directly for downstream applications such as qPCR, or any kind of enzymatic reaction.
Features
Optimized for (automated) use on PurePrep 24
Suitable for use with fresh or frozen plasma, serum or urine samples
Plasma can be collected with various blood collection tubes (Streck Cell-Free DNA BCT, EDTA, Citrate etc.)
Kit provides reagents for 96 extractions of cfDNA from 2 mL sample
Scalable for use between 1 and 4 mL sample, support protocol for 10 mL sample volume available
Typical yield: 0.5 to 4 ng cfDNA per mL of human plasma (but highly variable from donor to donor)
Following lysis at 56°C all other steps are processed at RT
Does not require carrier RNA