FFPE stands for “Formalin-Fixed Paraffin-Embedded,” and it refers to a common method of preserving and storing biological tissue samples for histological analysis and molecular studies. FFPE tissue is widely used in pathology, research, and clinical diagnostics.
This kit is intended for manual and automated isolation of genomic DNA from mammalian FFPE tissue or cell samples. Processing time for the preparation of 96 samples is about 40 minutes plus an additional paraffin removal step and lysis incubation.
Safety
The kit does not require phenol/chloroform extraction or alcohol precipitation and eliminates the need for repeated centrifugation, vacuum filtration or column separation. It allows safe handling of potentially infectious samples, and is designed to avoid sample-to-sample cross-contaminations. The obtained DNA can be used directly for downstream applications such as PCR, NGS, or any kind of enzymatic reaction.
Features
Short and easy protocols, 96 samples in 40 minutes
Multiple options for deparaffinization
Eliminates need for repeated centrifugation, vacuum filtration or column separations
Consistently high yield and purity of genomic DNA
Suitable for many downstream applications
Depending on the sample materials RNA may be co-purified. If required RNase treatment has to be integrated in the purification protocol. RNase is not included in the kit (available separately)
Easy to automate
PurePrep/ KingFisherTM/ BioSprint 96/ MagMaxTM protocols and consumables available
Compatible with general liquid handling robots (e.g. Hamilton®, TECAN®)
Magnetic separators for microtubes and microplates separately available for convenient manual or automated DNA extractions