Wire myograph IS-05

for preclinical researchfor cardiac musclesin vitro

MDE's small vessel myograph setups are specifically designed for investigating blood vessels under isometric conditions, with internal diameters ranging from 100 μm to 1 mm—dimensions too small for conventional organ baths. These vessels are prepared as ring structures by threading them onto two stainless steel or tungsten wires, securely fastened to supports. One support is connected to a micrometer, enabling precise control of vessel circumference, while the other is linked to a force transducer for tension measurement. The entire setup is housed in a chamber filled with physiological salt solution at 37°C, ensuring optimal conditions. Vessels in our Wire Myographs remain viable for several hours. During experiments, vessel circumference is maintained constant, and they are observed under isometric conditions. Compounds are introduced directly into the chamber, and vessel tension is continuously monitored. This setup allows for the assessment of vessel reactivity to various compounds and facilitates comparison between vessels from patients or test groups and those of controls, in terms of both reactivity and morphology.