Protease reagent MH458-01

proteinase Kfor researchfor histology

Storage Temperature: Stored at room temperature for three years. Product Introduction: Proteinase K is a non-specific serine protease, and the RNase and DNase activities have been removed after purification. Proteinase K is active in the presence of SDS or urea as well as in a wide range of pH, salt concentration and temperature, and can be increased in 1% SDS. Proteinase K is widely used in the preparation of pulsed electrophoresis chromosomal DNA, western blot and genomic DNA. The working concentration is usually 50-500g/mL, and the specific working concentration is determined according to whether the buffer solution contains SDS, urea, pH, temperature and other factors. Product Nature: RNase and DNase are not included; Specs:1g; Active in denaturant, such as urea and SDS; Active in metal chelators, such as EDTA; Active in a wide pH range (pH 4-12.5), the best pH range is pH 7.5-8.0; Active in a wide range of temperature, generally operating temperature range of 50℃-65℃; ddH2O can be dissolved; 7.Enzyme activity value: ≥30 U/mg Lyophilized powder. Method of Inactivation: After heating at 95℃-98℃ for 10 min, or adding PMSF or DFP inhibitor into the reaction system, it was deactivated. Remark: The general storage concentration is 10-20 mg/ml to avoid repeated freeze-thaw; Ca2+ can increase the thermal stability of protease K.