For the immunomagnetic enrichment and staining of circulating epithelial cells in peripheral blood. Cells are captured using anti-EpCAM antibodies bound to ferrofluid.
Fluorescent staining reagents are then added for identification of circulating epithelial cells.
Anti-CK-PE specific for the intracellular protein cytokeratin (epithelial cells), DAPI stains the cell nucleus, and anti-CD45-APC to identify leukocytes.