The Ampli1 WGA QC Kit is a multiplex PCR-based assay providing a useful quality control check of the WGA procedure.
By testing for the presence of one short and three long DNA fragments in the WGA library, the input DNA integrity and suitability of the WGA amplification product for further downstream analyses can be assessed.
Cells with good quality DNA typically produce 3 or 4 PCR bands, while fixed cells or cells with degraded DNA will show fewer QC bands. The number of bands is useful to define a “Genome Integrity Index” (GII) for predicting success of various downstream analytical methods and for assessing the quality of the starting DNA.
Product is for research use only (RUO) – not for use in diagnostic procedures.